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Lab Practicum Review

1. Identify the bacterial organelle shown.  What is its function?  Give the gram reaction.

  • Organelle: Capsule.
  • Function: Cytotoxic.
  • Gram reaction: Indeterminate because it’s not a gram stain.

2. Name the plant pathogen shown. What is its significance?

  • Pathogen: Claviceps purpurea.
  • Significance? Potent hallucinogen used in religious ceremonies / Causes ergotism / Predisposes to gas gangrene (pick one of these).

3. Liver biopsy of a marine recently returned from Afghanistan.  Identify the organism genus/species, disease and vector.

  • Organism. Leishmania Donovanii.
  • Disease.  Leishmaniasis / Dum dum fever / kala-azar
  • Vector: Brown Sand Fly Vector

4. Monogamous female with a week long history of a foul smelling creamy discharge. Identity? Give the stage. How is it acquired?

  • Identity: Trichomonas Vaginalis
  • Stage: Trophozoite; No cyst stage, also motile due to presence of flagella.
  • Acquisition: Sexual Transmission

5. Blood smear of a native from Sudan with a 3 week history of recurring fever. Give the organism genus/species and vector.

  • Plasmodium malariae
  • Disease: Malaria
  • Vector: Female anopheles mosquito.

6. Name the parasitic flatworm shown. Give the common name and where in the world is this expected to be found.

  • Identity: Clonorchis Sinensis.
  • Where is this found? Asia (EE’s backyard)
  • Common name: Asian Liver Fluke

7. Name the Structure shown, name the organism from where this is taken and how is it typically acquired?

  • Scolex structure.
  • Organism: Taenia solium.
  • From undercooked pork/wild game

8. Name the colonargea shown.  What stage is it? What is its significance?

  • Colonargea volvox.
  • Stage: Zygospore.
  • Significance? Important evolutionary basis for multicellularity.

9. Identify the common fungus shown.  Give the stage. Significance?

  • Fungus: Rhizopus spore
  • Stage: Zygospore stage.
  • Significance: Opportunistic mycosis in compromised host.

10. Name the common environmental fungus shown. What is its significance?

  • Fungus: Aspergillus.
  • Significance: Causes lung/ear infections (black ear disease; very common in farmers) / Aphylatoxin is produced. opportunistic mycosis in compromised host.

11. Found in normal flora in gut.

  • Candida albicans.
  • Fungal vaginitis (yeast infection); diaper rash; esaphagitis in AIDS patients.

12. Name the fungus shown, what is its significance?

  • Penicillium
  • Rarely associated with infection.

13. Name the parasitic flatworm, give the common name, how is it acquired?

  • Genus/species: Fasciola hepatica
  • Common name: Sheep liver fluke
  • Acquired by contaminated game.

14. Blood smear of a native from wherever.  Name the organism, vector and disease.

  • Trypanosoma organism
  • African sleeping sickness
  • Vector: tse-tse fly.

15. Gram stain of a 25 year old promiscuous male who thinks only wimps wear condoms.

  • Neisseria Gonorrhea.
  • Diasese: Gonorrhea / The Clap / French toilet
  • Transmission: STD

16. Name the bacterial organelle show. Give the function. Suggest the identity based on the characteristic morphology.

  • Organelle: Flagella
  • Function: Motility
  • Organism: Proteus Vulgaris

17. Trichrome stain of a volunteer who returned from Ecuador with bloody diarrhea.  Identify the organism, give the stage, associated disease and how is it acquired.

  • Organism: Entamoeba Histolytica
  • Stage: Trophozoite
  • Disease: Amebiasis
  • Acquisition: Fecal contaminated water.

18. Muscle biopsy from a football player fed smoked mountain lion by his coach. Name the organism, disease and how is this acquired.

  • Organism: Trichinella Spiralis
  • Disease: Trichinosis
  • Acquired: Contaminated/undercooked meat/game

19. Trichrome stain of a 2 year child who is not potty trained with foul smelling, explosive diarrhea.  Give the organism, stage, disease and acquisition.

  • Organism: Giardia lamblia
  • Stage: Cyst because it has four nuclei
  • Disease: Giardiasis (Beaver fever)
  • Acquisition: fecal contaminated water.

20. Name the bacterial organelle shown taken from a facultative bacteria. Give the gram reaction and how do you know this.

  • Organelle: Endospore.
  • Gram reaction: Positive because only gram positive bacteria form spores.
  • Likely identity: Bacillus

21. Identify the bacterial structure. Suggest the likely identity since this is from an obligate anaerobe.

  • Organelle: Endospore.
  • Gram reaction: Positive
  • Organism: Clorstridium perfringens.

22.  A gram positive, obligate anaerobic organism was isolated from the necrotic tissue of this truck driver. Identify the organism, diagnose the disease.

  • Organism: C. perfringens.
  • Disease: Gas gangrene

23. Fingers and back of a hairdresser 2 days after surgery from a broken hip. A gram positive, coagulase positive, catalase positive organism was isolated from the blood.  Identify the organism, diagnose the disease, what’s unusual if it is?

  • Organism: S. aureus.
  • Disease: Post operative toxic shock.
  • This is common in women and unusual in men.

23. Trichrome stain from 2 year old with explosive diarrhea. (compare this with #19!)

  • Organism: Giardia lamblia.
  • Stage: Trophozoite
  • Disease: Giardiasis (Beaver fever)
  • Acquisition: Fecal contaminated water

24. Name the pattern hemolysis show and one organism given this pattern.

  • Hemolysis: Beta.
  • Organisms: C. perfringens / S. aureus. / S. agalactiae / Strep pyogenes.

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